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Briefly, cartilage slices were digested with 0.25% trypsin (Lonza, Belgium) for 30 minutes and then incubated for 46 h in Dulbeccos modified Eagles medium/F-12 (DMEM/F-12

3, 2005, pp

A successful stem cell or cell-free product should be matched to the timing and metabolic phenotype of injury, manufactured with potency assays linked to its intended mechanism, and evaluated with biomarkers that show target engagement before functional outcomes are interpreted

Narwal R, Akhlaghi F, Asberg A, Hermann M, Rosenbaum SE

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