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The membranes were incubated with primary antibody (mouse anti-GLP-1R, sc-390774, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000 and rabbit anti-GAPDH, sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000) in TBST overnight at 4 C
Some protocols recommend intramuscular injection into the worked muscle ("site injection"), with the theory that local IGF-1R stimulation drives localized hypertrophy
The IADVL position is explicit that the infusion must be physician- supervised with pre-treatment screening for hepatic, renal, and G6PD status
Ensure adequate protein intake (1.6-2.2g per kg of body weight)
Continuing Therapy & Maintenance Phase Once the desired weight loss is achieved, the dosage may be further adjusted to maintain results
HUBERMAN: Now the pineal makes other things besides melatonin
