Presented for research literature review only Coles B et al
GLP-1 stands for glucagon-like peptide-1, a naturally occurring hormone your body releases after eating
Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations
[15] Safety check: If you must use compounded medication, work with a healthcare provider and verify that the compounding pharmacy is a state-licensed 503A or 503B facility
They should also ask about possible side effects, contamination risks and interactions with other medicines
Uh, if you have any questions about GLP-1 agonists that popped into your head after losing this episode, send them in