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For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio
When IGF-IR is deleted in mature osteoblasts, using OCN promoter-driven Cre line, the KO mice produce less trabecular bone, with an increased proportion of osteoid in the bone, indicating impaired mineralizing functions in OCN IGF-IR / osteoblasts (58,59)
G., Salam, A., Rai, B., Baskar, A., Dhawan, A., & Jomy, M
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