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The other oxidized form with a lower mobility most likely represents a glutathionylated protein species (strep-MBP-GRXC2-SG)

For crystallization of ShHTL4, 0.40 L of protein solution (18.0 mg mL 1 ) was mixed with 0.40 L of reservoir solution containing 90 mM Tris (pH 9.0), 180 mM MgCl 2 , 27% (w/v) PEG4000, and 1 mM GSH and incubated at 20 C

GHK-Cu has been studied extensively for its role in modulating copper transport and its downstream effects on collagen and elastin synthesis, antioxidant defence, and tissue remodelling pathways in laboratory settings

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