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Data are represented as mean SEM
Smit, T
Available at: (Accessed: 27th August 2015)
MTX and HPE-treated group Rats were injected with MTX (5 mg/Kg b.w) intraperitoneally for five consecutive days and at the same time, HPE (10.08 mg/Kg b.w) subcutaneously daily for two weeks
Immunoblots Cells or tissues were lysed in buffer (150 mM NaCl, 10 mM Tris pH 7.6, 0.1% SDS and 5 mM EDTA) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, 78445)
These actions protect gut tissue from damage and support optimal digestion and nutrient absorption
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