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For cloning purposes, cells were grown in lysogeny broth (LB) medium (10 g L 1 tryptone, 5 g L 1 yeast extract, and 10 g L 1 sodium chloride) at 37C on a shaking incubator (shaking frequency: 180 min 1 , eccentricity: 25 mm)

Nutr Res Rev, 2018

During GLP-1associated weight loss, lean-mass changes are typically adaptive and preventable , not degenerative. GLP-1 Body Composition Changes: What Clinical Trials Show About Muscle Loss Clinical trials and body-composition sub-studies of semaglutide and tirzepatide consistently demonstrate: Fat mass accounts for most weight lost during treatment. Lean mass may decrease , particularly without resistance training or adequate protein intake. In many patients, the proportion of lean mass relative to total body weight is preserved or improved , because fat loss exceeds lean loss. Key clinical insight: GLP-1/GIP therapies improve overall body composition by preferentially reducing fat massnot by causing muscle breakdown. Lean mass refers to all the non-fat tissues in the bodyprimarily skeletal muscle , but also organs, bones, connective tissue, and body waterthat support metabolism, movement, and overall physical function

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Individuals with Cardiovascular Risk Factors Some studies suggest that GLP-1 shots may have cardiovascular benefits beyond their effects on blood sugar control and weight management

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