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Blood from the tail vein was collected from baseline (prior to gavage), 10 min, 1, 2, and 3 h after gavage in ethylenediaminetetraacetic acid (EDTA)-coated capillary tubes for total GLP-1 and GIP measurements at baseline and 15 min post-gavage

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Hopkins published the complete structure in 1935, establishing that the thiol (-SH) group on cysteine was the reactive site responsible for the molecule's reducing properties

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