cDNA was synthesized from 1 g of total RNA using the RevertAid TM First Strand cDNA synthesis kit (Thermo Fisher Scientific, Monza, Italy) according to the manufacturers instructions
Treatment duration varies by indication and injury severity
Their findings have not yet appeared in a peer-reviewed journal
The cells were then pretreated with epigallocatechin-3-gallate (EGCg) prior to irradiation
PMID 18644225
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