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The beneficial effect of TZT in T2DM and obesity is related to the direct activation of GIP and GLP-1 receptors with subsequent improvement of insulin sensitivity and reduction of bodyweight (Chavda et al
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A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)
