Further studies tell us that both injection and intranasal administration of DSIP effectively pass the blood brain barrier, significantly increasing the cortical delta activity (delta waves)
May mix to taste with water, juice, or smoothie
TB-500 (5 mg Vial): Standard / Gradual Approach (2 mL = 2.5 mg/mL) Reconstitute: Add 2.0 mL bacteriostatic water to the TB-500 (5 mg) vial 2.5 mg/mL
After 18 h incubation at 27C, excess media was removed from the wells and replaced with 100 L of solution C containing either 9 10 3 (1 MOI) or 2.7 10 4 (3 MOI) zoospores per well for 1 hr, after which the inoculation solution as removed and replaced with 100 L solution A (25, 28)
Duan X, Liu R, Zhao Y, Geng Y, Xi Y, Tian Z
Collected cell pellets from 1 mL cultures were resuspended in 400 L of methanol at -80 C to quench the sample