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Editing efficiency was determined on bulk-edited cell populations by targeted PCR of the Cas9-targeted region and secondary PCR using overhang sequences (Table S1), followed by Illumina MiSeq, as previously described (Aubrey et al, 2015)
Am J Hosp Palliat Care
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Paradise lost: the neurobiological and clinical consequences of child abuse and neglect
The World Health Organization (WHO, 1991) estimates that 8-12% of couples worldwide experience some forms of infertility during their reproductive lives, thus affecting 50-80 million couples, with 20-30 million in Africa
The reduced form is the most bio-available and the common dosage is 100 to 200 mg per day
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