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One hour later, the filter paper was collected, and the abnormal image analysis software (Molecular equipment) was used to scan and calibrate each urine spot, and the urine spot area was measured
The libraries were quantified with a Qubit fluorometer (Life Technologies) and sequenced on Illumina Novaseq with 150-bp paired-end reads
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doi: 10.1016/j.autrev.2025.103864
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