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Cells were lysed in NP40 buffer and protein was quantified using Pierce BCA Protein Assay Kit (Sigma)
The saline base of the infusion replenishes fluids at the cellular level, making it ideal for combating dehydration caused by illness, travel, or intense exercise
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NNMT uses up nicotinamide by tagging it with a methyl group (a process called methylation) and sending it out as waste
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