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In addition, the activation of GLP-1R results in a cascade that activates adenyl cyclase via G s , resulting in an increased secretion of cyclic adenosine monophosphate (cAMP) secretion and activating cAMP-dependent protein kinase (PKA)

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For TUNEL staining, 40 l of equilibration buffer was added on each section for 30 s after the hydrogen peroxidase quenching step