Suitable fractions were pooled, concentrated with Amicon Ultra 10 kDa filters (Merck Life Science Ltd, Gillingham, UK) and diluted in 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl and 5 M urea buffer for storage at -80 C
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In addition to protecting cells against the destructive effects of free radicals and detoxifying harmful substances such as drugs and environmental pollutants, it has also been studied for the treatment of a number of serious medical conditions including Parkinsons Disease, heart disease, kidney disease, HIV and cancer
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Colloid Interface Sci
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