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Four-colour imaging with TRaQ-G and roGFP HeLa cells were plated and co-transfected with ERHalomGold and roGFP-iE-ER (3:1)

Insomnia model establishment The insomnia model was established by PCPA at a dose of 300 mg/kg via i.p

Activity regulated by mechanical stress, hormones (GH, IGF-1, PTH), local growth factors (BMPs, TGF-), and inflammatory signals

Eshdat et al

Non-randomised studies were rated as 7 stars classified as low risk, 36 stars as moderate risk, and 02 stars as high risk of bias, based on selection, comparability, and outcome assessment domains (Table 6)