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Presented for research literature review only Wan Y et al

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and step 3: stirring to dissolve and clarify the prepared solution, adding water for injection to full amount, and adjusting pH to 6.8 with 10% sodium hydroxide solution

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In this system, Atg4, Atg7, and Atg3 mediate the conjugation of soluble LC3 (microtubule-associated protein 1 light chain 3) to phosphatidylethanolamine (PE), producing LC3-II