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The functionality of the differentiated HLCs was rigorously assessed using several in vitro assays
The shRNA sequence (TRCN0000322995) targeting ATG10 was designed according to MISSION shRNA Plasmid DNA (MERCK) and synthesized and joined the FastDigest BshTI/FastDigest EcoRI linearized (Thermo Fisher) pLKO.1-GST DAF vector with T4 DNA ligase (EL0011, Thermo Fisher)
They rapidly raise blood B12 levels by bypassing the gut
The trade-off involves doubling the number of injections required
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