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Although this view was later attenuated based on the head-to-head conformation of the DNA-bound DBD dimer that was observed in X-ray crystallography studies and observations of relaxed response element stringency for selective chromatin binding [51, 52], it did point to the potential of variability in the composition of AREs and to deviations from the ideal 15 bp sequence
Song, W
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cDNA was amplified using a real-time PCR machine (QuantStudio, Thermo Fisher Scientific) with primers shown in Table S9
Each batch carries a unique COA covering all four components
Using data from the European Registration of Congenital Anomalies and Twins (EUROCAT) database, a case-control study, involving 596 cases and 2,359 controls, found that consumption of at least 400 g/day of folic acid during the periconceptual period (one month before conception through eight weeks' post-conception, covering the period of embryonic heart development) was associated with an 18% reduced risk of congenital heart defects (59)
