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This led to the development of new drugs similar to GLP-1 but with a longer-lasting effect

The scoring standard for IHC staining adopted the immunoreactive score (IRS) system [15, 16], with the specific rules as follows: the staining intensity of GGCT protein was classified into four grades, where a score of 0 indicated no staining signal, 1 indicated weak positivity, 2 indicated moderate positivity, and 3 indicated strong positivity

It was first characterized by a Croatian research group in the 1990s studying gastroprotective peptides, and what made it interesting was that the protective effects extended far beyond gut tissue

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Most published rodent studies use 10 micrograms per kilogram body weight administered subcutaneously or intraperitoneally