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*JAMA*, 281(15), 1415-1423

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After an initial wash in 0.1 M PBS (pH 7.4), the tissues were preincubated in a solution of 3% normal goat or donkey serum and 0.5% Triton X-100 in 0.1 M phosphate-buffered solution (PB, pH 7.4) for 30 min to block non-specific binding

E., Magyar, P