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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

Hospira Pfizer Bacteriostatic Water for Injection, USP is a sterile aqueous solution used for diluting, dissolving, or reconstituting medications and compounds that require sterile water