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Antibodies used were 1:1000 rabbit anti-IBA1 (Wako, 019-19741), 1:500 rabbit anti-CD45 (Abcam, ab214437), and 1:1000 donkey anti-rabbit Alexa Fluor 488 (Invitrogen, A-21206)

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Rodent studies consistently document 4060% increases in food intake following GHRP-6 administration, depending on model and dose