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Researchers must adhere to established protocols to maintain the integrity of their data

Before proceeding the experiment calibration of IRGA was done that includes zeroing replacement of drierite and soda lime

After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter

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