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Metabolic extraction Approximately 30,000 adult worms or 5 mL of culture medium per sample were processed

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Ethics declarations Acknowledgements Medical writing support in the preparation of this manuscript was provided by Susie Eaton, MBio, and Tom Priddle, DPhil, of OPEN Health Communications (UK), funded by Boehringer Ingelheim

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And what we're targeting are naturally occurring gut hormones

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All mixtures were vortexed for 10 min to ensure thorough mixing and protein precipitation, followed by centrifugation at 15,000 rpm for 10 min

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David DC, Hauptmann S, Scherping I, Schuessel K, Keil U, Rizzu P, et al

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