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Subsequently, the cells were washed with HBSS buffer, prior to adding CYS (20 M) and the ROS inducer menadione (50 M) as positive control, along with the staining solution (HBSS, calcium, magnesium, 5 M MitoSOX red and 10 g/mL Hoechst (Thermo))

Sperm retrieval is an essential part of all assisted reproductive techniques