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MRL/lpr mice A mouse strain with an Fs gene mutation (lpr) leading to defective lymphocyte apoptosis and lupus-like symptoms

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To overexpress the wild-type enzyme and its variants, 10 mL of an overnight culture (16-18 hours) was inoculated into 1 L of Luria-Bertani broth supplemented with 50 mg/mL kanamycin and 34 mg/mL chloramphenicol

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