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Thirty or forty micrograms of proteins were loaded into each well and fractionated on 510% precast SDS-PAGE gels (Byeotime, Shanghai, China) and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany)
The GLP-1 drugs seemed to work across the board
The peripheral changes in fat metabolism are a downstream consequence of reduced energy intake rather than a direct pharmacological target
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