Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)
Funding This study was supported by Natural Science Foundation of Guangdong Province (2021A1515012393), NSFC grant (82371692, 32170825), Key laboratory of functional and clinical translational medicine, Guangdong Provincial Sports Bureau Scientific Research Project (GDSS2024N007), and Guangdong Medical Science and Technology Research Foundation (A2023145)
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Clinical trials indicate that it takes approximately one to three months of continuous supplementation to achieve statistically significant elevations in deep tissue stores and to observe measurable improvements in immune cell cytotoxicity
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Further analysis using Western blotting indicates that BPC-157 may activate the FAK (focal adhesion kinase) and paxillin proteins