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The seven clusters were designated as follows: ECM-regulatory NPCs (ECM-reg NPCs), homeostatic NPCs (Hom-NPCs), adhesion NPCs (Adh-NPCs), effector NPCs (Eft-NPCs), hypertrophic NPCs (Ht-NPCs), fibrotic NPCs (Fibro-NPCs), and regulatory NPCs (Reg-NPCs)

The resulting mixture is electroporated with 200,000 cells in electroporation buffer and the cells are deposited into antibiotic-free medium, as described above, and allowed to rest in a 37 C incubator for 24 h before additional antibiotic-containing medium was added