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The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added
The problem with natural GLP-1 is that it is short-lived
Peer et al
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It provides dosage adjustments tailored to your unique response
This is what makes them convenient for patients, one injection per week is much more manageable than daily injections, but it also creates the timing questions that do not arise with shorter-acting peptides
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