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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

Do not rinse after application

BPC-157 peptide appears to bind with growth hormone receptors, possibly stimulating cell proliferation

Laboratory investigations have explored its interactions with hypothalamic and limbic receptor systems, as well as its potential modulatory effects on stress hormone pathways in preclinical settings

"Protein Synthesis: From Ribosomes to Post-Translational Modifications"

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