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Stained membranes were then washed with TBST and stained with corresponding secondary antibodies in 5% milk in TBST for one hour

The standard method requires trickling a compatible solvent, like bacteriostatic water, slowly down the inside glass wall of the vial, followed by gentle hand-swirling to prevent fracturing the molecular bonds

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Salem EA, et al

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Compared with traditional methods, DL-based algorithms provide promising prospects for enhanced biomarker detection and precision oncology, but further validation is needed for better integration with real-world clinical work