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The chemical co-trimoxazole we used in the study was water soluble, most of the chemical was supposed to remain in the culture supernatant, proper treatment of the supernatant can help lower the risk

The soreness correlates with injection technique: reviews mentioning "slow injection over 30 seconds" report 28% less soreness than those describing "quick push" technique (pattern analysis from 340 reviews specifying technique)

These include the complexity of data analysis, patient heterogeneity, timing of sample collection, and accessibility to high-quality tissue samples from critically ill patients

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Liu J, Kang R, Tang D

Palomba S, Santagni S, La Sala GB