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Whole cell lysates from hemin-treated WT HeLa cells (b) and isolated mitochondrial lysate from cells treated with 5-ALA and FAC (c) were used as positive controls, respectively

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Establish a systematic rotation pattern, such as: Week 1: Right abdomen Week 2: Left abdomen Week 3: Right thigh Week 4: Left thigh Week 5: Right upper arm Week 6: Left upper arm Week 7: Return to right abdomen This approach ensures each site receives adequate recovery time between injections

Eur J Pharm Biopharm 100:6676