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Bacterial V3 region of 16S rRNA gene sequencing Biopsies were thawed, weighed (1050 mg) and homogenized in 700 L Qiazol lysis buffer (Qiagen, Hilden, Germany) using a Pro200 tissue homogeniser (PRO Scientific, Oxford, CT, USA)

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Zinc deficiency also widely present in autistic children further worsens copper excess, since zinc and copper compete for the same transport pathways