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A 60 L solution of 1.5 M potassium phosphate buffer (KPi, pH 7.4), NaN 3 2 mM and 3-(trimethylsilyl)propionic-2,2,3,3- d4 acid (TSP) 0.16 mM in D 2 O was added to 540 L of supernatant sample
Krivic A., Anic T., Seiwerth S., Huljev D., Sikiric P
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After incubation, 1 mL of absolute EtOH was added and SP3 beads were magnetically captured and washed three times with 80% EtOH, then resuspended in 200 L 50 mM HEPES buffer (pH 7.4) containing 1 mM TCEP and incubated for 3 min at RT
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