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The results you record won't be attributable to the dose you thought you were testing
With neurological symptoms: 1000 g intramuscularly on alternate days should be continued for up to three weeks or until there is no further improvement
It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile
In my experience, injections in the skeletal muscles (intramuscular injections) result in a more dramatic improvement in symptoms including energy and weight loss