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Recent technological advancements, such as in vivo labeling of newly synthesized RNAs with modified uridines like 4-thiouridine (s(4)U) or bromodeoxyuridine (BrdU), along with isolation of chromatin-bound transcripts, have enabled precise quantification of steady-state and newly synthesized RNA levels These approaches have been particularly instrumental in studying cells stimulated with LPS or TNF, elucidating the coordinated transcriptional and post-transcriptional networks that orchestrate immune responses