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2008 Jan;134(1):166-178

Angiogenesis Studies: Angiogenic potential investigated through multiple assays: HUVEC tube formation (network complexity), endothelial migration (cells/field), aortic ring sprouting (sprout length/number), VEGF/VEGFR2 expression (qPCR, Western blot), CAM assay (vessel density)

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Solvent choice, compatibility, final solution volume, handling, and storage must come from validated material-specific documentation