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Patented Fermentation Controlled fermentation designed to preserve glutathione integrity and stability

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This takes about 8 to 15 minutes

Stock solutions of test and reference compounds were diluted to generate two working solutions: Solution A (0.5 mM): 10 l of a 10 mM DMSO stock diluted with 190 l of DMSO Solution B (0.02 mM): 8 l of solution A added to 192 l of phosphate buffer (final DMSO 4%) Working solutions were spiked into plasma by adding 20 l of solution B to 380 l of blank plasma in each well of a 96-well plate (final compound concentration: 1 M, 0.2% DMSO)

Cells were centrifuged and resuspended in 0.1 M sodium cacodylate buffer and post-fixed for 1 h with 1% osmium + 0.1 M sodium cacodylate buffer, then dehydrated and embedded in Agar 100 resin