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One hundred microliter aliquot of tissue homogenate supernatant was first incubated with 50 L KOH (1 mol/L) at 37 C for 30 min to fully hydrolyze the combined carnitine, and 10 L HCl (1 mol/L) was then added to neutralize the solution (sample A)

Nikfarjam, S., Rezaie, J., Zolbanin, N

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Structure determination was carried out using a ThermoFisher Titan Krios TEM operating at 300 kV equipped with a Gatan BioQuantum energy filter (with a 15 eV slit width for OCTN2 inward, OCTN2 ipratropium and a 25 eV width for OCTN2 carnitine), and a Gatan K3 camera

MT-2 is studied for its interaction with melanocortin receptor systems, which are involved in a range of biological signaling pathways including pigmentation biology, appetite-related signaling, energy balance, and neuroendocrine activity