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Spectra were background-corrected by subtraction of the spectrum obtained in the presence of microsomes and NADPH, respectively intact HepG, but in absence of NFT
PMID 21722960
Assay: Glutathione in rat tissue
The protein that results from the use of the downstream UGA stop codon, due to readthrough translation, is identified as VDRAx
The -cyano amide-based Michael acceptors have also been elegantly used by the Taunton group as warheads in small-molecule inhibitors to react with active site cysteines while being minimally affected by the high concentration of intracellular GSH due to the fast reaction kinetics and the mM of K d (refs 31, 32)
PMID: 24667154
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