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The nucleophile provides the electrons to form the new bond and the pi bond of the carbonyl group is broken as it "gets out of the way." The electrons move from this pi bond onto what was the carbonyl oxygen

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Contains berberine is not a mechanism

Ultrasensitive FRET-based aptasensor for interleukin-6 as a biomarker for COVID-19 progression using nitrogen-doped carbon quantum dots and gold nanoparticles