sedebokerense -infected algal culture completely abolished the parasitism, suggesting that methionine biosynthesis was essential for the fungal infection [40]
This phase includes sulfation (adding a sulfo group), glucuronidation (adding glucuronide), glutathione conjugation (pairing), acetylation (adding an acetyl group), amino acid conjugation, and methylation
The resolved gels were transferred from the pre-cast SDS-PAGE gels to polyvinylidenefluoride (PVDF) membranes, and blocking was performed with 5% bovine serum albumin (BSA) in Tris-buffered saline (TBS-T) containing 0.05% Tween-20 and incubated with the primary antibodies: MOTS-c antibody (MOTSC-101AP, FabGennix)
Negative controls were prepared by omitting the primary antibodies
Those questions will usually tell you which peptide family the research is really pointing toward, even if the headline lumps them together
The conservative cutoff is the labeled date