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Library preparation and next-generation sequencing Lysates were homogenized and denatured at 95 C for 5 min, and equal lysate volumes from each treatment well were added to a custom PCR master mix for PRISM cell barcode amplification

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Because many previous reports demonstrated that hypertension alone induces a shift from fatty acid oxidation toward increased glucose utilization in cardiac muscle, hypertension complicated with HFpEF might have additive effects on energy metabolic failure in heart

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