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Urban N, Tsitsipatis D, Hausig F, Kreuzer K, Erler K, Stein V, et al

Transfer volumes are given in millilitres and microlitres (1 mL = 1000 L)

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Then sections were incubated with Mouse-on-Mouse blocking reagent for 1 hour at room temperature (RT) to block the endogenous immunoglobulins following overnight at 4C incubation with specific primary antibodies targeting different myosin heavy chain (MHC) isoforms: MHC IIa (DHSB, SC-71) and MHC IIb (DHSB, BF-F3) with 2.5% goat serum in 1X PBS

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A provider should assess these biomarkers before starting semaglutide to determine whether B12 addition is clinically justified for that individual