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elegans orthologue of human adh-5 gene (H24K24.3) was knocked out using the CRISPR/Cas9 system as previously reported: The preassembled CRISPR/Cas9 ribonucleoprotein complexes and linear single stranded DNAs as repair templates were directly injected into the gonad of young adult hermaphrodites 61

J Inorg Biochem 98: 16341641