Indeed, CFTR upregulation was shown to lead to increased apoptosis in endometrial epithelial cells, as well as to abnormal fluid accumulation within the uterine lumen, hence disrupting the supportive environment needed for successful embryo implantation [7, 33,34,35]
From a drug development perspective, the key research question is how the screened drug candidates bind to their target proteins to produce their pharmaceutical effects
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Then, the membranes were blocked with 5% skim milk and incubated overnight at 4 C with primary antibodies against SLC25A39 (1:2000, Novus, cat